http://localhost:8080/xmlui/handle/123456789/1043 2026-01-21T00:56:35Z 2026-01-21T00:56:35Z Dr.Karishma M Desai http://localhost:8080/xmlui/handle/123456789/1253 2022-12-22T08:36:11Z 2022-01-01T00:00:00Z

Title: Effect of arecoline and transforming growth Factor on myofibroblast transdifferentiation By assessment of mitochondrial bioenergetics A cell culture study Authors: Dr.Karishma M Desai Abstract: Background: Oral sub mucous fibrosis (OSMF) is a condition wherein mesenchymal response to injury are mediated through changes in fibroblast structure and function. Fibroblasts differentiate to alpha smooth muscle actin positive (αSMA+) myofibroblast, which is key feature underlying pathogenesis of OSMF. Studies highlight that myofibroblast phenotypes vary from physiological to pathological conditions. Recently, role of transforming growth factor β (TGFβ) and mitochondrial reprogramming has been studied in organ fibrosis. In OSMF, the role of arecoline and TGFβ is well-recognized but there is a paucity in literature regarding their effect in myofibroblast differentiation and mitochondrial function. Thus, the present work aimed to evaluate the role of arecoline and TGFβ in myofibroblast differentiation by study of mitochondrial bioenergetics. Objectives: Primary Objective: To evaluate role of arecoline and TGFβ on myofibroblast transdifferentiation using human oral fibroblast cell lines. Secondary Objectives: 1. To assess the effect of arecoline, TGFβ and combination of arecoline & TGFβ on myofibroblast transdifferentiation. 2. To assess the mitochondrial bioenergetics in myofibroblast transdifferentiation induced by arecoline, TGFβ and combination of arecoline and TGF β. 3. To compare the mitochondrial bioenergetics in myofibroblast transdifferentiation induced by arecoline, TGFβ and combination of arecoline and TGFβ.

2022-01-01T00:00:00Z Dr.Ritiha S Patil http://localhost:8080/xmlui/handle/123456789/1252 2022-12-22T08:34:45Z 2022-01-01T00:00:00Z

Title: Assessment of effect of curcumin On arecoline induced human buccal Mucosal fibroblasts a cell culture Study Authors: Dr.Ritiha S Patil Abstract: Introduction: Oral cavity is lined by the oral mucous membrane that is made up of an overlying epithelial layer and an underlying connective tissue. Fibroblasts are the principle cells of the oral mucosa and buccal mucosa which in normal condition secrete collagen and elastin fibres. In pathological condition fibroblasts actively take part in wound healing. An abnormal increased secretion of collagen (Fibrosis) by the cells is noted in both systemic and oral diseases. One of the common conditions associated with fibroblast pathologies is Oral sub mucous fibrosis (OSMF). OSMF is a multi-factorial pathological disorder in which arecanut plays a major role for the pathogenesis of the precancerous condition affecting the fibroblasts at both cellular and molecular level. Despite many treatment modalities like cessation of habit surgical excision, diathermy, the disease still remains irreversible .Recent clinical studies have proved that the use of herbal products like curcumin, neem, tulsietc. have improved the signs and symptoms of OSMF. As curcumin is considered a safe natural product by Food and Drug Administration (FDA), it becomes a choice of herbal product in ourstudy. Aims of the study: Our study aims to isolate the buccal mucosal fibroblasts from healthy individuals and assess the effect of arecoline, curcumin and curcumin on arecoline induced cells for morphological and cytotoxic alterations.

2022-01-01T00:00:00Z Dr. Sankeshwari Roopali Manohar http://localhost:8080/xmlui/handle/123456789/1086 2021-10-12T09:59:46Z 2019-01-01T00:00:00Z

Title: “Effectiveness of Licorice varnish, Fluoride varnish and Combination of Both, on Incipient Lesions of Early Childhood Caries in 3-4 year old preschool children of Belgaum city-A randomized controlled trial” Authors: Dr. Sankeshwari Roopali Manohar Abstract: Background – Dental caries is widespread oral health problem with prevalence reaching as high as 80% in some places. Even infants and toddlers are not spared and when this younger group gets infected with caries it is called Early Childhood Caries. Modern medicine, especially fluoride is known for caries prevention since early 1980’s. With rejuvenating interest in herbal medicine, the present research focused on Licorice, root of traditional medicinal plant. Literature so far is affirmative about role of licorice in oral health promotion. Aim – To compare and evaluate effectiveness of indigenously prepared Licorice varnish, Combination varnish with fluoride varnish on initial lesions of Early childhood caries and their antibacterial activity against S.mutans. To assess if parental Locus of Control is associated with caries experience of their children.

2019-01-01T00:00:00Z Dr. Sidramesh Muttagi http://localhost:8080/xmlui/handle/123456789/1085 2021-10-12T09:46:54Z 2019-01-01T00:00:00Z

Title: “Pattern of cervical lymph node metastasis And its association with clinico-pathological Variables in primary mandibular gingivobuccal Sulcus squamous cell carcinoma” Authors: Dr. Sidramesh Muttagi Abstract: Background: Squamous cell carcinoma (SCC) of mandibular Gingivo-Buccal Complex (GBC) or “Indian Oral Cancer,” is a distinct cancer of oral cavity in the Indian subcontinent. Cervical lymph node metastasis (CLNM) is a validated prognostic factor in mandibular GBC-SCC like other subsites of oral SCC. CLNM in oral SCC is known to occur in a foreseeable manner, involving mainly level I, level II and to lesser extent level III nodes unlike tongue or floor-mouth SCC wherein, the CLNM tends to be extensive. The available guidelines for neck dissection (ND) in node-positive necks for oral SCC are based on western studies, which comprise more of tongue and floor-mouth SCC and lack patients with mandibular GBC-SCC. Also, the literature about accurate nodal staging in Indian patients with mandibular GBC- SCC is scanty with regards to adequacy of lymph-nodes to be harvested following ND. Furthermore, the extent of CLNM in advanced oral SCC is influenced by clinico- pathological factors that have to be identified for risk stratification with regards to decision making on the extent of ND in patients with mandibular GBC-SCC. This will help surgeons to practice oncologically safe ND for patients with high risk of extensive CLNM and contrarily to perform limited-extent ND in low risk patients, thus avoiding morbidities related to comprehensive ND. Aim and Objectives: To study the metastatic involvement of cervical lymph-nodes (CLNs) at various levels in the neck from primary mandibular GBC-SCC and quantify the number of lymph-nodes harvested from comprehensive ND. To study the contribution of clinico-pathological factors like T-size, histological differentiation (HD), infiltration of bone by the tumor, lympho-vascular tumor emboli (LVE) and perineural Invasion (PNI) in CLNM.

2019-01-01T00:00:00Z