Assessment of effect of curcumin On arecoline induced human buccal Mucosal fibroblasts a cell culture Study

Abstract

Introduction: Oral cavity is lined by the oral mucous membrane that is made up of an overlying epithelial layer and an underlying connective tissue. Fibroblasts are the principle cells of the oral mucosa and buccal mucosa which in normal condition secrete collagen and elastin fibres. In pathological condition fibroblasts actively take part in wound healing. An abnormal increased secretion of collagen (Fibrosis) by the cells is noted in both systemic and oral diseases. One of the common conditions associated with fibroblast pathologies is Oral sub mucous fibrosis (OSMF). OSMF is a multi-factorial pathological disorder in which arecanut plays a major role for the pathogenesis of the precancerous condition affecting the fibroblasts at both cellular and molecular level. Despite many treatment modalities like cessation of habit surgical excision, diathermy, the disease still remains irreversible .Recent clinical studies have proved that the use of herbal products like curcumin, neem, tulsietc. have improved the signs and symptoms of OSMF. As curcumin is considered a safe natural product by Food and Drug Administration (FDA), it becomes a choice of herbal product in ourstudy. Aims of the study: Our study aims to isolate the buccal mucosal fibroblasts from healthy individuals and assess the effect of arecoline, curcumin and curcumin on arecoline induced cells for morphological and cytotoxic alterations.