Effect of arecoline and transforming growth Factor on myofibroblast transdifferentiation By assessment of mitochondrial bioenergetics A cell culture study

Abstract

Background: Oral sub mucous fibrosis (OSMF) is a condition wherein mesenchymal response to injury are mediated through changes in fibroblast structure and function. Fibroblasts differentiate to alpha smooth muscle actin positive (αSMA+) myofibroblast, which is key feature underlying pathogenesis of OSMF. Studies highlight that myofibroblast phenotypes vary from physiological to pathological conditions. Recently, role of transforming growth factor β (TGFβ) and mitochondrial reprogramming has been studied in organ fibrosis. In OSMF, the role of arecoline and TGFβ is well-recognized but there is a paucity in literature regarding their effect in myofibroblast differentiation and mitochondrial function. Thus, the present work aimed to evaluate the role of arecoline and TGFβ in myofibroblast differentiation by study of mitochondrial bioenergetics. Objectives: Primary Objective: To evaluate role of arecoline and TGFβ on myofibroblast transdifferentiation using human oral fibroblast cell lines. Secondary Objectives: 1. To assess the effect of arecoline, TGFβ and combination of arecoline & TGFβ on myofibroblast transdifferentiation. 2. To assess the mitochondrial bioenergetics in myofibroblast transdifferentiation induced by arecoline, TGFβ and combination of arecoline and TGF β. 3. To compare the mitochondrial bioenergetics in myofibroblast transdifferentiation induced by arecoline, TGFβ and combination of arecoline and TGFβ.