Evaluation of the effects of ranolazine on Chronic inflammation in male wistar rats

Abstract

Introduction & Objective: Inflammation is the main cause for the most of diseases; it may acute or chronic in nature. Chronic inflammation type of diseases needs to take medications long time, which is totally not curable and for controlling limited medications available with greater adverse reactions. The piperazine derivative of Ranolazine shows effect on acute and sub-acute inflammation. The current experimental study was planned with the primary objective to evaluate the effects of drug Ranolazine on Chronic inflammation in male Wistar rats. The secondary objective was to study the effect on inflammatory markers like TNF-α, IL-1β, IL-6 and CRP. Materials and Methods: Chronic inflammation induction by 2 types of models was used: 1. Cotton pellet and Grass pith. (Foreign body induced granuloma) 2. Complete Freund’s adjuvant (CFA) induced chronic inflammation. (Rat paw edema) Test animals were divided as 3 groups (n=6) in each model, drugs given per orally vehicle, aspirin and ranolazine in clinically equivalent dose. In rats cotton pellets and grass piths induction was done by subcutaneously, (Model-1) and CFA injection at right hind limb (Model-2). After induction every 24hours drug was given to animals up to 21 days. The rats were sacrificed to obtain cotton pellets and grass piths. Mean granuloma dry weight of cotton pellets for various groups was measured and percentage inhibition of granuloma dry weight was calculated. Post study period blood samples collected and estimated the serum inflammatory markers. The grass pith sections were stained with haematoxylin and eosin for histopathological studies. CFA induced rats; hind limb paw edema was measured by plythesmometer on 1 day of study. Post study period blood samples collected and evaluated the serum inflammatory markers and joints dissected and histopathological studies done.