Development of new Ayur Probiotic Formulation and its evaluation in Experimental animals

Abstract

In the present era, the concept of “PROBIOTICS” is inspiring in food and pharmaceutical industry. Probiotics are live bacteria that, if consumed in adequate amounts, provide the host with some health benefits. Probiotics like Lactobacillus and Bifidobacterium are most frequently utilized. Probiotics have been linked to anti- infection properties, a decrease in serum cholesterol, relief from lactose intolerance, and an improved balance of the intestinal microbial flora, which can treat diarrhoea in all its forms, including antibiotic-associated diarrhoea (AAD), traveller’s diarrhoea, diarrhoea brought on by infection (both bacterial and viral forms), constipation, the synthesis of vitamins, and nutritional enhancement. About 5–30% of individuals get antibiotic-associated diarrhoea (AAD), either at the beginning of their antibiotic medication or for as long as two months after it has ended. So, one such drug in Ayurveda named Bilwa (Aegle marmelos. Linn), especially about its tender young fruit (apakwa bilwa phala ie,) which possesses katu (bitter), tikta (pungent), kashaya (astringent), teekshna, snigdha guna, ushna veerya and finest samgrahika (capable of absorbing fluids from the gastrointestinal tract), deepaniya (appetisers) and pacifies kapha and vata dosha 9,10,11 . Apakwa bilwa phala is having marmelosin, luvangetin, aurapten, psoralen, marmelide & tannin as an active ingradients. With considering all these supportive evidences, one such study is planned to develop “Bilwaphala saara dadhi” an Ayur-probiotic preparation which includes apakwa bilwaphala majja in dadhi media. And the same preparation will be used for evaluating its effect in Antibiotic Associated Diarrhoea (AAD) in the Wister rats. Aim:  Development and efficacy study of an Ayur-Probiotic (Bilvaphalasaara dadhi) preparation xii

Objectives: • To develop an Ayur-Probiotic preparation and assessment of its bacterial load. • To evaluate the efficacy of Ayur-Probiotic in Antibiotic associated diarrhoea in experimental animals.