Comparative Evaluation of the Effect of Different Final Irrigation Regimens 1:1 Mixture of 3% NAOCL+18% HEDP and 17% EDTA on the Dentinal Tubule Penetration of two different root canal sealers: A Confocal Laser Scanning Microscopic Study

Abstract

ABSTRACT Aim- To evaluate the effect of 17% EDTA and 1:1 mixture of 3% NaOCl+18% HEDP as final irrigating solution on the maximum depth of penetration of an epoxy resin-based sealer (AH Plus) and BIOACTIVE GLASS sealer into the dentinal tubules at the apical areas of root canals using Confocal Laser Microscopic Study. Methodology: Eighty extracted human mandibular premolar teeth with single root and single canal were selected. They were disinfected in 0.1% thymol solution, cleaned of calculus and soft tissues and stored in 0.1% thymol solution till use. All teeth were radiographed and selected as per the inclusion and exclusion criteria. The teeth were decoronated using a diamond disk under copious water spray to acquire a standardized root length of 14 mm. Working length was established by inserting a size 10 K file into each root canal until it is visible at the apical foramen and by subtracting 1mm from the recorded length. Instrumentation of the root canal was done till master apical file size of F2/F3 using ProTaper universal, rotary instruments. The canals were irrigate with 2 mL of 3% sodium hypochlorite between successive files. Teeth were randomly divided in 4 subgroups n= 20 according to the intervention. Passive ultrasonic irrigation was used to activate the irrigants. Final irrigation was performed with distilled water. The teeth were obturated using AH plus sealer and Bioactive glass sealer coated with rhodamine dye. Depth of sealer penetration into dentinal tubules was assessed using Confocal Laser Scanning Microscope