Dentinal tubule penetration of a Silicone-based endodontic sealer Following n- acetyl cysteine intracanal Medicament removal using ultrasonic Agitation and laser-activated Irrigation- an in- vitro study.

Abstract

ABSTRACT Aim and Objectives: To evaluate and compare the dentinal tubule penetration of a silicone-based endodontic sealer following N- acetyl cysteine intracanal medicament removal using Ultrasonic Agitation and Laser-Activated Irrigation- an In- vitro study. Study design: Eighty one extracted human mandibular premolar single rooted teeth were selected, disinfected and decoronated to obtain a standardized root length of 12 mm. The teeth were prepared with ProTaper Universal rotary files upto MAF F3. PREPARATION OF INTRACANAL MEDICAMENT: N-acetyl cysteine powder was mixed with propylene glycol in the ratio of 1:1. N- acetyl cysteine intracanal medicament placed using a size #30 Lentulospiral until visible at the apical foramen. The orifice was sealed with Cavit, and specimens stored in an incubator at 37°C with 100% humidity for 14 days. After the incubation period, the specimens were instrumented with #30 Hedström files supplemented with 5 mL of 3% NaOCl to remove the medicament. Then, the specimens were divided into three groups according to irrigant activation techniques for medicament removal: Group 1: Diode laser activation Group 2: Passive Ultrasonic agitation Group 3: No agitation (positive control) The canals were rinsed with 5ml of saline solution and dried with paper points and were prepared for obturation. GuttaFlow bioseal sealer was with 0.1% Rhodamine B dye. The canals were obturated with gutta-percha cones in combination with the GuttaFlow bioseal sealer using a single cone technique. Teeth were then sealed with vi Cavit and incubated at 37°C and 100% humidity for a week to simulate clinical conditions. The specimens were sectioned horizontally using a diamond disc to obtain 1 mm thick sections from 2, 5 and 8-mm levels from the apex. Sections were examined under confocal laser scanning microscope to measure depth of sealer penetration (in μm) into the dentinal tubules using Image J software