The effect of Probiotics on Aspirin induced Gastric Mucosal Lesion (Gastric ulcer) in comparison to Pantoprazole in male Wistar rats - An Experimental Study

Abstract

ABTRACT INTRODUCTION : Aspirin, a Globally used NSAIDS drug , is meant for its analgesic, antipyretic, and antiplatelet benefits . However, its long-term use is associated with gastric mucosal lesion and ulcer formation, primarily due to cyclooxygenase-1 inhibition, which decrease protective PGs synthesis. The outcome because of its decreased in mucus and bicarbonate release, increased gastric acid formation, oxidative stress, and inflammation, helpful in mucosal erosion and improving ulcer healing. Proton pump inhibitors , like pantoprazole, are the routine treatment for Non – steroidal antiinflammatory drugs-induced gastric leasion, as they inhibit gastric acid release and improving ulcer healing. However, long term Proton pump inhibitors use has been involved with gut microbiota alteration , rebound increased acid , and disturbed nutrient absorption. Due to these apprehension, probiotics have emerged as a potential adjunctive therapy for gastric ulcer treatment , given their ability to modify gut microbiota, improving mucosal safty and apply, anti-inflammatory impact . The study intended to contrast the gastrointestinal safety effects of probiotics and pantoprazole in an aspirin-treated gastric ulcer screening in male Wistar rats by evaluating inflammatory cytokine levels, histopathological changes, and mucosal integrity. OBJECTIVES The primary goal of the study are:  Estimate probiotic effect on gastric mucosal injury induced by treatment of aspirin in male Wistar rats.  Differentiate the effects of probiotics with Pantoprazole for gastroprotective , a widely used PPIs , escape from aspirin-treated mucosal damage. Secondary Objective Investigate how probiotics affect the value infllammatory cytokines, particularly TNF-alpha, interleukin -2, and interleukin -4.