effect of lisinopril and perindopril In sodium nitrite and ethanol induced anterograde amnesia in male wistar rats

Abstract

Objective: The present study was planned to study the effect of lisinopril and perindopril in sodium nitrite and ethanol induced oxidative stress and apoptotic model of anterograde amnesia in male Wistar rats. Materials and Methods: Nine groups (n=10), of healthy male Wistar rats (150-180 grams) were used for the study. The induction of anterograde amnesia was done by sodium nitrite and ethanol. The paradigm used was Morris water maze where a hidden platform was kept for the rats to escape to. The rats were trained to locate a hidden platform by releasing them into water for four times a day for four consecutive days. The acquisition of this task was measured by noting the time taken to escape to the platform. On the fifth day of the study, retrieval of this learnt task was measured by noting their time taken to search for the missing hidden platform. These two data measured were then compared amongst test groups and amnesia induced groups. Data was expressed as mean ± standard error of mean. On the 5th day (Retrieval trial) only vehicle (distilled water i.p) was administered to the groups. Results: Lisinopril and perindopril completely ameliorated the induction of anterograde amnesia by sodium nitrite. Perindopril showed significant decreased in mean ELT in ethanol induced anterograde amnesia but lisinopril did not show any significant difference in respect to ethanol. Conclusion: The study drugs in the present study ameliorated chemical hypoxia induced anterograde amnesia pointing towards a possible anti-oxidant role of them. These drugs can therefore potentially inhibit oxidative stress induced neurodegeneration especially in the ageing population at the commonly prescribed clinical doses. Additionally, perindopril may be exerting antiapoptotic action that might attribute to more beneficial effect in amnesia. Further studies are needed to substantiate these findings.